Record number :
732393
Title of article :
Nucleotide sequencing of a polyurethanase gene (pulA) from Pseudomonas fluorescens
Author/Authors :
Carmen Ruiz، نويسنده , , Gary T. Howard، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Pages :
5
From page :
127
To page :
131
Abstract :
Nucleotide sequencing of a gene (pulA) encoding an extracellular polyurethanase/esterase from Pseudomonas fluorescens revealed an open reading frame encoding a 48 kDa protein of 451 amino acid residues. The deduced polypeptide sequence contained a serine-hydrolase consensus sequence GXSXG. The polypeptide lacked an N-terminal signal peptide, but did contain a short region toward the C-terminus that has been observed in secreted lipases, EXXXGXTFIIGSXGNDXIXGGXGXDXXEXXXGXD. The most closely related proteins to the polyurethanase had amino acid sequence homologies of 71%, 67%, 69%, 67% for lipases from P. fluorescens, and 51% for lipases from Serratia marcescens.
Keywords :
Polyurethanase , gene , Regulation , sequence
Journal title :
International Biodeterioration and Biodegradation
Serial Year :
1999
Link To Document :
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