The present study was designed to identify the volatile constituents across the oestrous cycle of bovine in order to detect
oestrous-specific chemical signal. The bovine saliva was extracted with diethyl ether (1 : 1 ratio, v/v) and analysed by gas
chromatography-linked mass spectrometry. Numerous compounds were identified during oestrous cycle of bovine saliva. Among
these, the compounds, namely, trimethylamine, acetic acid, phenol 4-propyl, pentanoic acid and propionic acid were specific to
oestrous stage. The behaviour assay revealed that the compound, trimethylamine, is involved in attracting the male animal.
The result concludes that the trimethylamine is considered as a putative oestrous-specific salivary chemo-signal in the bovine
cows , oestrus , Pheromones , Saliva , volatile compounds