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Title of article :
Highly sensitive and selective fluorescence optosensor to detect and quantify benzo[a]pyrene in water samples
Author/Authors :
Carretero، A. Segura نويسنده , , Fernandez-Sanchez، J. F. نويسنده , , Cruces-Blanco، C. نويسنده , , Fernandez-Gutierrez، A. نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
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Abstract :
We describe here a method for detecting and quantifying the highly carcinogenic polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP) in water, based on a flow-trough optical sensor. The technique is fast (response time of 40 s) and simple and at the same time meets the standards of sensitivity and selectivity required by the European Guidelines on Water for Human Consumption. The optosensor is based on the on-line immobilization of BaP on a non-ionic resin (Amberlite XAD-4) solid support in a continuous-flow system. BaP was analyzed in a 15 mM H2PO4-/HPO42- buffer solution with 25% (v/v) 1,4-dioxane at pH 7. Fluorescence intensity was measured at (lambda)ex/em=392/406 nm. The experimental conditions (reagent phase, pH, type and concentration of buffer solution and organic solvent) and flow-injection values (flow rate and injection volume) were carefully controlled. Under these conditions the optosensor was sensitive to a linear concentration range of between 3.0 and 250.0 ng l^-1 with a detection limit of 3.0 ng 1^-1 and a standard deviation of 1.5% at 150 ng l^-1. The optosensor was applied to the quantification of BaP in drinking and waste water samples (95–105% recovery) in presence of the other 15 EPA PAHs at 1000 ng l^-1 concentration level. The influence of other fluorescent polycyclic aromatic hydrocarbons and potential interference from ions usually present in water was also evaluated.
Keywords :
POLYCYCLIC AROMATIC HYDROCARBONS , water analysis , Fluorescence sensor
Journal title :
Analytica Chimica Acta
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