Ryunosuke Ohkawa، نويسنده , , Yuji Hirowatari، نويسنده , , Kazuhiro Nakamura، نويسنده , , Shigeo Ohkubo، نويسنده , , Hitoshi Ikeda، نويسنده , , Mitsumasa Okada، نويسنده , , Minoru Tozuka، نويسنده , , Kazuhiko Nakahara، نويسنده , , Yutaka Yatomi، نويسنده ,
Platelet release of α granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated.
Concentrations of the CXC chemokines β-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method.
β-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4°C were necessary to accurately measure β-TG and PF4, but not serotonin.
Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
PLASMA , Serotonin , Platelet factor 4 (PF4) , h-thromboglobulin (h-TG)