Record number :
2555380
Title of article :
Challenges in Culturing Macaca fascicularisBone Marrow Stem Cells (Cabaran di dalam Pengkulturan Sel Stem Sum-Sum Tulang Macaca fascicularis)
Author/Authors :
Aini, Sharen, S. Universiti Kebangsaan Malaysia - Faculty of Medicine - Tissue Engineering Centre, Malaysia , Ng, M.H. Universiti Kebangsaan Malaysia - Faculty of Medicine - Tissue Engineering Centre, Malaysia , Shamsul, S.B. Universiti Kebangsaan Malaysia - Faculty of Medicine - Tissue Engineering Centre, Malaysia , Ruszymah, B.H.I. Universiti Kebangsaan Malaysia - Faculty of Medicine - Tissue Engineering Centre, Malaysia , Masfueh, R. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Periodontology, Malaysia , Badiah, B. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Periodontology, Malaysia , Chua, K.H. Universiti Kebangsaan Malaysia - Faculty of Medicine - Department of Physiology, Malaysia , Low, C.K. Universiti Kebangsaan Malaysia - Faculty of Medicine - Laboratory Animal Resource Unit, Malaysia , Nazimi, A.J. Mohd. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Oral and Maxillofacial Surgery, Malaysia , Rafiqah, N. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Oral and Maxillofacial Surgery, Malaysia , Ramli, R. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Oral and Maxillofacial Surgery, Malaysia , Norziha, Y. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Prosthodontics, Malaysia , Shariffal Shuriana, A.S. Universiti Kebangsaan Malaysia - Faculty of Dentistry - Department of Prosthodontics, Malaysia , Siar, C.H. university of malaya - Oral Medicine and Periodontology Faculty of Dentistry - Department of Oral Pathology, Malaysia
From page :
419
To page :
422
Abstract :
Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction
Keywords :
Bone marrow stem cells , Macaca fascicularis , tissue engineering
Link To Document :
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