Title of article :
The Effects of Exercise on Expression of CYP19 and StAR mRNA in Steroid-Induced Polycystic Ovaries of Female Rats
Aghaie, Fatemeh Department of Physiology - Faculty of Life Science and Biotechnology - Shahid Beheshti University, Tehran , Khazali, Homayoun Department of Physiology - Faculty of Life Science and Biotechnology - Shahid Beheshti University, Tehran , Hedayati, Mehdi Cellular and Molecular Research Center - Research Institute for Endocrine Sciences - Shahid Beheshti University of Medical Sciences, Tehran , Akbarnejad, Ali College of Physical Education and Sports Sciences - University of Tehran
Background: Polycystic ovarian syndrome (PCOS) is the most frequent female endocrine disorder that affects
5-10% of women. PCOS is characterized by hyperandrogenism, oligo-/anovulation, and polycystic ovaries. The
aim of the present research is to evaluate the expression of steroidogenic acute regulatory protein (StAR) and
aromatase (CYP19) mRNA in the ovaries of an estradiol valerate (EV)-induced PCOS rat model, and the effect of
treadmill and running wheel (voluntary) exercise on these parameters.
Materials and Methods: In this experimental study, we divided adult female Wistar rats that weighed approximately
220 ± 20 g initially into control (n=10) and PCOS (n=30). Subsequently, PCOS group were divided to
PCOS, PCOS with treadmill exercise (P-ExT), and PCOS with running wheel exercise (P-ExR) groups (n=10
per group). The expressions of StAR and CYP19 mRNA in the ovaries were determined by quantitative real-time
reverse transcriptase polymerase chain reaction (qRT-PCR). Data were analyzed by one-way ANOVA using SPSS
software, version 16. The data were assessed at α=0.05.
Results: There was significantly lower mRNA expression of CYP19 in the EV-induced PCOS, running wheel and
treadmill exercise rats compared to the control group (P<0.001). Treadmill exercise (P=0.972) and running wheel
exercise (P=0.839) had no significant effects on CYP19 mRNA expression compared to the PCOS group. mRNA
expression of StAR in the ovaries of the PCOS group indicated an increasing trend compared to the control group,
however this was not statistically significant (P=0.810). We observed that 8 weeks of running wheel and treadmill
exercises could not statistically decrease StAR mRNA expression compared to the PCOS group (P=0.632).
Conclusion: EV-induced PCOS in rats decreased CYP19 mRNA expression, but had no effect on StAR mRNA expression.
We demonstrated that running wheel and moderate treadmill exercise could not modify CYP19 and StAR
Cytochrome P450 Family 19 , Valerate , Exercise , Polycystic Ovarian Syndrome , Steroidogenic Acute Regulatory Protein
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