Baniasadian ، Shabnam - Islamic Azad University, Ahar Branch , Farajnia ، Safar - Tabriz University of Medical Sciences , Jafari ، Behboud - Islamic Azad University, Ahar Branch
Genome-wide association studies in Asian population for the first time identified common variants in the KCNQ1 gene to be associated with type 2 diabetes in Japanese populations, recently. This result has been replied in some other East Asian population but, still now, there is no any report about this subject in Iranian population. The aim of this study was to investigate the frequency of KCNQ1 variants in type 2 diabetes in East Azerbaijan population, northwest of Iran. A total of 75 T2D and 90 normal East Azerbaijan subjects were entered the study. A demographic date was recorded for all subjects. Genomic DNA was extracted from the whole blood, and the KCNQ1 single nucleotide polymorphisms (SNPs) rs2237892 were genotyped with PCR-RFLP technique. The results of the study indicated an increased level of FBS and HbA1C among T2D cases (P 0/0001), as expected. Screening for KCNQ1 rs237892 among T2D case group revealed that the genotype frequencies of CC, CT and TT were 87.0% (67/77), 10.4% (8/77) and 2.6% (2/77), respectively. The allelic frequencies of C and T in case subjects were 92.21% (142/154) and 7.79% (12/154), respectively. Analysis of the normal control subjects showed the genotype frequencies of CC, CT and TT were 94.4% (85/90), 3.3% (3/90) 2/2% (2/90), respectively. The allelic frequencies of C and T among controls were 96.11% (173/180) and 3.89% (7/180), respectively. In conclusion, the results of our study indicated that there are no any significant differences in frequency of KCNQ1 rs237892 alleles between T2D and control subjects.
Type 2 diabetes , GWAS , KCNQ1 rs237892 , Polymorphism