Record number :
2434210
Title of article :
Novel Single Nucleotide Polymorphisms (SNPs) in Two Oogenesis Specific Genes (BMP15, GDF9) and Their Association with Litter Size in Markhoz Goat (Iranian Angora)
Author/Authors :
Arefnejad, B. Department of Animal Science - Faculty of Agriculture - University of Shiraz, Shiraz, Iran , Mehdizadeh, Y. Department of Animal Science - Faculty of Agriculture - University of Shiraz, Shiraz, Iran , Javanmard, A. Department of Animal Science - Faculty of Agriculture - University of Tabriz, Tabriz, Iran , Zamiri, M.J. Department of Animal Science - Faculty of Agriculture - University of Shiraz, Shiraz, Iran , Niazi, A. Department of Biotechnology - Faculty of Agriculture - University of Shiraz, Shiraz, Iran
Pages :
9
From page :
91
To page :
99
Abstract :
BMP15 and GDF9 are two oogenesis specific genes play a pivotal role in female fertility in mammals and potential for improvement of prolificacy in marker-assisted selection. The aim of present research was to investigate the variation and association between BMP15 and GDF9 polymorphism and litter size in Mark-hoz goats. The sequence variability of the different amplified fragments utilized for genotyping was further investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), poly-merase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing. Sequencing technology allowed the detection of two nucleotide variations: A both non-functional mutation (G/A) tran-sition at nt 1026 known as P3) and a synonymous mutation at 1049 position which for the first time was found at the BMP15 gene. Also, GDF9 gene exhibited a polymorphism on BcnI site (1159 bp) within exon 2 of amplified PCR products (glutamine/proline). Our analysis using general linear model (GLM) statistical methods showed litter size was significantly influenced by genotypes of GDF9 (P<0.01). Heterozygous genotype showed higher litter size than homozygous genotypes (P<0.01). The conformation of this found-ing would need large sample size and test to screen in other indigenous goats.
Keywords :
BMP15 , fecundity , GDF9 , polymorphism
Journal title :
Astroparticle Physics
Serial Year :
2018
Link To Document :
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