Record number :
2384516
Title of article :
Optimization of Three Dimensional Culturing of the HepG2 Cell Line in Fibrin Scaffold
Author/Authors :
Banihashemi، Mehrzad نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Banihashemi, Mehrzad , Mohkam، Milad نويسنده Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran , , Safari، Azam نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Safari, Azam , Nezafat، Navid نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Nezafat, Navid , Negahdaripour، Manica نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Negahdaripour, Manica , Mohammadi، Fatemeh نويسنده , , Kianpour، Sedigheh نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Kianpour, Sedigheh , Ghasemi، Younes نويسنده Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran ,
Issue Information :
ماهنامه با شماره پیاپی 0 سال 2015
Pages :
1
From page :
0
To page :
0
Abstract :
A potential treatment for healing hepatic tissue is delivering isolated hepatic cells to the site of injury to promote hepatic cells formation. In this technology, providing an appropriate injectable system for delivery of hepatic cells is an important issue. In this regard, fibrin scaffolds were designed with many advantages over other scaffolds like cell delivery vehicles for biodegradation, biocompatibility and hemostasis. The aim of this study was to determine suitable cell culture circumstances for HepG2 cell proliferation and differentiation in 3D fibrin scaffolds by evaluating Ca concentrations, cell numbers, various ratios of plasma/RPMI 1640 and thickness of fibrin scaffold. In a one-stage experimental design, Box-Behnken design strategy was performed by Minitab 15 software (version 15, Minitab. State College, PA) with three factors at three levels (low, medium and high) and 27 runs for identification of the effects of ratio of plasma/RPMI 1640, Ca concentration and thickness on the formation of fibrin gel scaffold and 3D HepG2 culture. The optimal concentrations for fibrin scaffold fabrication were achieved by adding 0.15 mol CaCl (50 µL) and 1 × 10 cells to 1:4 of plasma/RPMI 1640 ratio (500 µL with 2.3 mm thickness per well). Our approach provided easy handle method using inexpensive materials like human plasma instead of purified fibrinogen to fabricate fibrin scaffold.
Journal title :
Hepatitis Monthly
Serial Year :
2015
Link To Document :
بازگشت