Record number :
2275741
Title of article :
Effect of illumination, casein hydrolysate and proline on callus induction of Oryza sativa L. CV. MR219
Author/Authors :
C.M.Z.، Che Radziah نويسنده Faculty of Science and Technology , , A.K.، Siti Nurkhalida نويسنده Faculty of Science and Technology , , Z.، Zamri نويسنده Faculty of Science and Technology , , I.، Ismanizan نويسنده Faculty of Science and Technology ,
Issue Information :
دوفصلنامه با شماره پیاپی سال 2012
Pages :
5
From page :
37
To page :
41
Abstract :
In this study, factors such as illumination, casein hydrolysate and proline were shown to produce high numbers of calli from MR219 mature seeds. The effect of different concentrations (1, 2, 3, 4 and 5 mgL-1) of 2,4-dicholorophenoxy acetic acid (2,4-D) under different illuminations (24 hours light, 16/8 hours photoperiod, dark) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (100%) and growth (90%) was observed in the Murashige and Skoog (MS) medium supplemented with 5 mgL-1 2,4-D incubated under 16/8 hours photoperiod. The effect of different concentrations of casein hydrolysate (0, 300, 400, 500, 600, 700, 80 and 1000 mgL -1) and proline (0, 500, 1000 and 1500 mgL-1) on callus induction and growth of MR219 were also studied. It was found that increasing concentrations of casein hydrolysate and proline did not give a significant effect on the percentage of callus induction. However, a significant percentage of callus growth (90%) was recorded when the callus induction medium was supplemented with 1000 mgL-1 proline.
Journal title :
Malaysian Applied Biology
Serial Year :
2012
Link To Document :
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