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Title of article :
Simple spectrophotometric methods for quantification of modafinil us-ing 1,2-naphthoquinone-4-sulphonate and 2,4-dinitrophenol as analytical reagents
Author/Authors :
ونكاتا سه شامامبا، بورلا سونيتا نويسنده Department of Food Chemistry and Nutrition, College of Food Science and Technology, Bapatla, Andhra Pra-desh-522101, India Venkata Seshamamba, Burla Sunitha , ونكاتا ساتيانارايانا، پروري ويرا نويسنده Department of Biochemistry, Acharya Nagarjuna University, Guntur, Andhra Pradesh-522510, India Venkata Satyanarayana, Peruri Veera , بالا سكاران، چاندرا نويسنده Department of Biotechnology, Jagarlamudi Kuppuswamy Choudary College, Guntur, Andhra Pradesh-522006, India Bala Sekaran, Chandra
Issue Information :
فصلنامه با شماره پیاپی سال 2014
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Abstract :
Two simple visible spectrophotometric methods are developed and validated for the quantifica-tion of modafinil using 1,2-naphthoquinone-4-sulphonic acid (NQS method) and 2,4-dinitrophenol (DNP method) as analytical reagents. The NQS method involves the reaction of modafinil with 1,2-naphthoquinone-4-sulphonate in alkaline medium at room conditions to form a yellow colored product exhibiting maximum absorption at 430 nm. DNP method is based on the proton transfer from 2,4-dinitrophenol to modafinil at room conditions and then we have the formation of yellow colored ion-pair complex exhibiting maximum absorption at 475 nm. Dif-ferent variables affecting the reaction were studied and optimized. Under the optimized experi-mental conditions, Beerʹs law is obeyed in the concentration ranges of 10-100 and 8-60 ?g/mL with the detection of limit values of 0.486 and 0.258 ?g/mL for NQS method and DNP method, respectively The molar absorptivity and Sandellʹs sensitivity for both of the methods are reported. The methods were validated in terms of accuracy, precision and robustness. The results were satisfactory. The proposed methods were effectively applied to the analysis of the modafinil in their tablet formulations. The recoveries were 99.92% and 99.96% with RSD and 0.863% and 0.722% for NQS and DNS methods, respectively. The assay was not interfered by common ex-cipients.
Journal title :
Iranian Chemical Communication
Journal title :
Iranian Chemical Communication
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