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Title of article :
Platycodi Radix attenuates dimethylnitrosamine-induced liver fibrosis in rats by inducing Nrf2-mediated antioxidant enzymes
Author/Authors :
Choi، نويسنده , , Jae-Ho and Jin، نويسنده , , Sun Woo and Kim، نويسنده , , Hyung Gyun and Khanal، نويسنده , , Tilak and Hwang، نويسنده , , Yong Pil and Lee، نويسنده , , Kyung-Jin and Choi، نويسنده , , Chul Yung and Chung، نويسنده , , Young Chul and Lee، نويسنده , , Young Chun and Jeong، نويسنده , , Hye Gwang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
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Abstract :
The purpose of this study was to investigate the anti-fibrotic effects of the aqueous extract of the Platycodi Radix root (Changkil: CK) on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. DMN treatment for 4 weeks led to marked liver fibrosis as assessed by serum biochemistry, histopathological examination, and hepatic lipid peroxidation and collagen content. CK significantly inhibited DMN-induced increases in serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, fibrosis score, and hepatic malondialdehyde and collagen content. CK also inhibited DMN-induced reductions in rat body and liver weights. Reverse transcription polymerase chain reaction (RT-PCR) and western blot analyses revealed that CK inhibited DMN-induced increases in matrix metalloproteinase-13 (MMP-13), tissue inhibitor of metalloproteinase-1 (TIMP-1), and tumor necrosis factor-α (TNF-α) mRNA, and collagen type I and α-smooth muscle actin protein. DMN-induced cyclooxygenase-2 (COX-2) expression and nuclear factor-kappa B (NF-κB) activation was reduced by CK treatment. Furthermore, CK induced activation of nuclear erythroid 2-related factor 2 (Nrf2)-mediated antioxidant enzymes such as γ-glutamylcysteine synthetase (γ-GCS), heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), and glutathione-S-transferase (GST) in HepG2 cells. These results demonstrated that CK attenuates DMN-induced liver fibrosis through the activation of Nrf2-mediated antioxidant enzymes.
Keywords :
Platycodi Radix , Antioxidant enzymes , Liver fibrosis , Nrf2 , COX-2
Journal title :
Food and Chemical Toxicology
Journal title :
Food and Chemical Toxicology
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