Record number :
Title of article :
Inhibition of acrolein-stimulated MUC5AC expression by Platycodon grandiflorum root-derived saponin in A549 cells
Author/Authors :
Choi، نويسنده , , Jae Ho and Hwang، نويسنده , , Yong Pil and Han، نويسنده , , Eun Hee and Kim، نويسنده , , Hyung Gyun and Park، نويسنده , , Bong Hwan and Lee، نويسنده , , Hyun Sun and Park، نويسنده , , Byung-Keun and Lee، نويسنده , , Young Chun and Chung، نويسنده , , Young Chul and Jeong، نويسنده , , Hye Gwang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
From page :
To page :
Abstract :
Mucin overproduction is a hallmark of chronic airway diseases such as chronic obstructive pulmonary disease. In this study, we investigated the inhibition of acrolein-induced expression of mucin 5, subtypes A and C (MUC5AC) by Changkil saponin (CKS) in A549 cells. Acrolein, a known toxin in tobacco smoke and an endogenous mediator of oxidative stress, increases the expression of airway MUC5AC, a major component of airway mucus. CKS, a Platycodon grandiflorum root-derived saponin, inhibited acrolein-induced MUC5AC expression and activity, through the suppression of NF-κB activation. CKS also repressed acrolein-induced phosphorylation of ERK1/2, JNK1/2, and p38MAPK, which are upstream signaling molecules that control MUC5AC expression. In addition, the MAPK inhibitors PD98059 (ERK1/2), SP600125 (JNK1/2), and SB203580 (p38 MAPK), and a PKC delta inhibitor (rottlerin; PKCδ) inhibited acrolein-induced MUC5AC expression and activity. CKS repressed acrolein-induced phosphorylation of PKCδ. Moreover, a reactive oxygen species (ROS) inhibitor, N-acetylcysteine, inhibited acrolein-induced MUC5AC expression and activity through the suppression of PKCδ and MAPK activation, and CKS repressed acrolein-induced ROS production. These results suggest that CKS suppresses acrolein-induced MUC5AC expression by inhibiting the activation of NF-κB via ROS–PKCδ–MAPK signaling.
Keywords :
Platycodon grandiflorum , Acrolein , Saponin , Muc5AC , NF-?B
Journal title :
Food and Chemical Toxicology
Journal title :
Food and Chemical Toxicology
Serial Year :
Link To Document :