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Title of article :
Molecular characterization of miiuy croaker CC chemokine gene and its expression following Vibrio anguillarum injection
Author/Authors :
Cheng، نويسنده , , Yuanzhi and Wang، نويسنده , , Ri-xin and Sun، نويسنده , , Yue-na and Xu، نويسنده , , Tian-jun، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
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Abstract :
A CC chemokine gene was isolated from miiuy croaker (Miichthys miiuy) by expressed sequence tag analysis. The Mimi-CC cDNA contains an open reading frame of 429 nucleotides encoding 142 amino acid residues. The deduced Mimi-CC possesses the typical arrangement of four cysteines as found in other known CC chemokines (C31, C32, C56, and C70). It shares 15.3%–37.4% identity to CC chemokines of mammal and teleost. Phylogenetic analysis showed that miiuy croaker was most closely related to Atlantic cod. Genomic analysis revealed that Mimi-CC gene consists of four exons and three introns, which is not typical of CC chemokines but resembles that of CXC chemokines. Real-time quantitative RT-PCR demonstrated that Mimi-CC is constitutively expressed in most tissues including lymphoid organs, and the highest expression of Mimi-CC transcripts in normal tissues was observed in muscle. Challenge of miiuy croaker with Vibrio anguillarum resulted in significant changes in the expression of CC chemokine transcripts in four tissues, especially in kidney and spleen.
Keywords :
Miiuy croaker , Gene expression , innate immunity , CC chemokine
Journal title :
Fish and Shellfish Immunology
Journal title :
Fish and Shellfish Immunology
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