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Title of article :
Genomic organization and mRNA expression of manganese superoxide dismutase (Mn-SOD) from Hemibarbus mylodon (Teleostei, Cypriniformes)
Author/Authors :
Cho، نويسنده , , Young Sun and Lee، نويسنده , , Sang Yoon and Bang، نويسنده , , In Chul and Kim، نويسنده , , Dong Soo and Nam، نويسنده , , Yoon Kwon، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
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Abstract :
The genomic structure of manganese superoxide dismutase (Mn-SOD) was characterized in Hemibarbus mylodon (Teleostei, Cypriniformes), an endangered freshwater fish species, and changes in Mn-SOD mRNA expression in response to various stimuli, such as challenge with lipopolysaccharide (LPS), bacteria, or heavy metals, were examined. H. mylodon Mn-SOD possesses well-conserved features of vertebrate Mn-SODs, including its homologous quinquepartite gene structure (five exons interrupted by four introns) and high sequence identity with vertebrate orthologs in coding regions, including a typical eukaryotic Mn-SOD signature. The 5′-regulatory upstream region of the H. mylodon Mn-SOD gene lacked a canonical TATA sequence; however, it displayed various transcription factor binding elements that may be involved in immune and stress responses, as well as the modulation of enzymes that mediate the metabolism of metals and other xenobiotics. Bioinformatic analysis of Mn-SOD genes from fish and mammalian genome databases revealed chromosomal synteny of the Mn-SOD locus in the vertebrate lineage. Mn-SOD transcripts were ubiquitously detected in diverse tissues with variable levels of basal expression using an RT-PCR assay. Mn-SOD expression in the liver and kidney were significantly modulated by injection of LPS (1, 5, or 10 μg g−1 body weight), Edwardsiella tarda challenge (5 × 103 or 5 × 105 cells/fish), and heavy metal exposure (Cd, Cu, or Zn at 5 μM). Results from this study suggest that Mn-SOD plays an important role in the host defence against oxidative damage caused by infection-mediated inflammation and/or toxicant related stress in this species.
Keywords :
Gene expression , Bacterial and LPS challenge , Hemibarbus mylodon , Mn-SOD , Heavy metal exposure , gene structure
Journal title :
Fish and Shellfish Immunology
Journal title :
Fish and Shellfish Immunology
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