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Title of article :
Molecular cloning and characterisation of cytosolic manganese superoxide dismutase (cytMn-SOD) from the giant freshwater prawn Macrobrachium rosenbergii
Author/Authors :
Cheng، نويسنده , , Winton and Tung، نويسنده , , Ying-Hsiu and Liu، نويسنده , , Chun-Hung and Chen، نويسنده , , Jiann-Chu، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
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Abstract :
A cytosolic manganese superoxide dismutase (cytMn-SOD) cDNA was cloned from the hepatopancreas of giant freshwater prawn Macrobrachium rosenbergii using reverse transcription polymerase chain reaction (RT-PCR) by degenerate primers. Both 3′- and 5′-regions were isolated by rapid amplification of cDNA end RACE method. Analysis of nucleotide sequence revealed that the cytMn-SOD cDNA clone consists of 1339 bp with an open reading frame of 858 bp encoding a protein of 286 amino acids. The calculated molecular mass of the mature proteins (286 amino acids) is 31 kDa with an estimated pI of 5.52. Two putative N-glycosylation sites, NXT and NXS were observed in the cytMn-SOD. Four conserved amino acids responsible for binding manganese were observed (H110, H158, D243 and H247). Sequence comparison showed that the cytMn-SOD deduced amino acid sequence of M. rosenbergii has an overall similarity of 77% and 54% to that of blue crab Callinectes sapidus and tiger shrimp Penaeus monodon, respectively. Quantitative real-time RT-PCR analysis showed that cytMn-SOD transcript in hepatopancreas of M. rosenbergii decreased 3 h after Lactococcus garvieae injection, but no significant change in cytMn-SOD transcript was observed in the haemocytes 3–24 h after L. garvieae injection.
Keywords :
Haemocyte , Macrobrachium rosenbergii , Lactococcus garvieae injection , Hepatopancreas , real-time RT-PCR , Cytosolic manganese superoxide dismutase (cytMn-SOD)
Journal title :
Fish and Shellfish Immunology
Journal title :
Fish and Shellfish Immunology
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