Record number :
2107124
Title of article :
Effects of dopamine on the immunity of white shrimp Litopenaeus vannamei
Author/Authors :
Cheng، نويسنده , , Winton and Chieu، نويسنده , , Hung-Tien and Tsai، نويسنده , , Chiung-Hui and Chen، نويسنده , , Jiann-Chu، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Pages :
11
From page :
375
To page :
385
Abstract :
The total haemocyte count (THC), phenoloxidase activity, respiratory burst, superoxide dismutase (SOD) activity, phagocytic activity and clearance efficiency in response to pathogen Vibrio alginolyticus were measured when the white shrimp Litopenaeus vannamei (20.0 ± 1.5 g) were injected individually with dopamine at 10−8, 10−7 and 10−6 mol shrimp−1, respectively. For the shrimp that received dopamine at 10−7 and 10−6 mol shrimp−1, the THC decreased by 25% and 39%, phenoloxidase activity decreased by 15% and 32%, respiratory burst decreased by 21% and 36%, and SOD activity decreased by 50% and 63%, respectively, after 4 h. The phagocytic activity and clearance efficiency of shrimp that received dopamine at either dose decreased significantly after 2 h. The THC, phenoloxidase activity, respiratory burst, SOD activity, phagocytic activity and clearance efficiency returned to normal values after 16, 8, 8, 24, 16 and 4 h, respectively, for the shrimp that received dopamine at either dose. In another experiment, L. vannamei which had received dopamine at 10−8, 10−7 and 10−6 mol shrimp−1 were challenged after 1 h by injection with V. alginolyticus at 1.0 × 105 colony-forming units (cfu) shrimp−1 and then placed in seawater of 20‰. The cumulative mortality of shrimp that received dopamine at either dose was significantly higher than that of shrimp that received saline after 8 h, and of shrimp that received saline at the termination of the experiment (48 h after the challenge). It is therefore concluded that dopamine administration at 10−6 mol shrimp−1 or less causes immune modulation of L. vannamei.
Keywords :
Litopenaeus vannamei , Dopamine , respiratory burst , Total haemocyte count , phenoloxidase activity , Clearance efficiency , Phagocytic activity , Superoxide dismutase activity
Journal title :
Fish and Shellfish Immunology
Journal title :
Fish and Shellfish Immunology
Serial Year :
2005
Link To Document :
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