Record number :
Title of article :
Regulation of anti-LDL immobilization on self-assembled protein G layer using CHAPS and its application to immunosensor
Author/Authors :
Choi، نويسنده , , Jeong-Woo and Park، نويسنده , , Ki-Suk and Lee، نويسنده , , Woochang and Oh، نويسنده , , Byung-Keun and Chun، نويسنده , , Bum-Suk and Paek، نويسنده , , Se-Hwan، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
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Abstract :
In antibody-based immunoassay, it is important to fabricate a structured base plate with high activity for antibody immobilization. To prevent the reduction of anti-LDL binding capacity due to protein G aggregation, detergent was introduced to control the size of protein G aggregate. The self-assembled monolayer of 11-mercaptoundecanoic acid (11-(MUA)) and hexanethiol mixture was fabricated to form the stable protein G layer. 3-[(cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate (CHAPS) was used for the regulation of protein G aggregate immobilized on the 11-(MUA) surface. The effect of various CHAPS concentrations on the amount of anti-low density lipoprotein (LDL) immobilized on protein G layer was investigated. Twelve millimolars of CHAPS were determined as the optimal concentration to maximize the binding capacity of anti-LDL due to the control of protein G density on the surface. The anti-LDL layer on self-assembled protein G using CHAPS was applied to surface plasmon resonance immunosensor for detection of LDL and its detection limit was 100 pM.
Keywords :
Protein G , CHAPS , Anti-LDL , Self-assembled monolayer , Immunosensor
Journal title :
Materials Science and Engineering C
Journal title :
Materials Science and Engineering C
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