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Title of article :
Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers
Author/Authors :
وجودي، الهام نويسنده گروه مامايي موسسه فناور يهاي نوين پزشکي مهر E, Vojoudi , ابراهيمي بيداسكان، وحيد نويسنده Dept. of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad Ebrahimzadeh-Bideskan , Vahid , فاضل ، عليرضا نويسنده ,
Issue Information :
فصلنامه با شماره پیاپی 0 سال 2015
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Abstract :
Background: The aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. Methods: Formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin histochemical method. In this study, two groups of horseradish peroxidase-labeled specific lectins were used: N-acetylgalactosamine, including Dolichos biflorus, Wisteria flori bunda agglutinin (WFA), Vicia villosa, Glycine max as well as focuse-binding lectins, including tetragonolobus, Ulex europaeus, and Orange peel fungus (OFA). All sections were counterstained with alcian blue (pH 2.5). Results: Our results showed that only WFA and OFA reacted strongly with the floor plate cells from early to late embryonic period of developing spinal cord. The strongest reactions were related to the 14, 15, and 16 days of tissue sections incubated with OFA and WFA lectins. Conclusion: The present study demonstrated that cellular and molecular differentiation of the spinal cord organizers is a wholly regulated process, and ?-L-fucose, ?-D-GalNAc, and ?/B-D-GalNAc termi nal sugars play a significant role during the prenatal spinal cord development. Iran. Biomed. J. 19 (1): 63-68, 2015
Journal title :
Iranian Biomedical Journal(IBJ)
Journal title :
Iranian Biomedical Journal(IBJ)
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