Choi، نويسنده , , Eun-Wha and Shin، نويسنده , , Il-Seob and Youn، نويسنده , , Hwa Young and Kim، نويسنده , , Dae-Yong and Lee، نويسنده , , Hang and Chae، نويسنده , , Young-Jin and Lee، نويسنده , , Chang-Woo، نويسنده ,
Although viral vectors are commonly used for therapeutic gene delivery, their applications are limited due to their specific cell membrane receptor-mediated infection and host immune response. In the present study, we constructed a non-viral peptide vector and applied it in the treatment of experimentally induced systemic lupus erythematosus-like disease in dogs. For therapeutic gene construction, the extracellular domain of canine CTLA-4, and the CH2–CH3 domains of canine immunoglobulin alpha constant region were inserted between the cytomegalovirus promoter and poly-adenylation sequence of bovine growth hormone. The constructed therapeutic gene was ligated to the non-viral synthetic peptide vector and was applied to systemic lupus erythematosus-like disease induced dogs. After gene therapy, clinical signs of systemic lupus erythematosus were reduced dramatically: the anti-nuclear antibody titers and urine protein/creatinine ratios were recovered to normal values, and the skin regained its normal histological features. The peptide vector did not show either tissue specific tropism or host induced immune response.
Non-viral peptide vector , CTLA-4 , systemic lupus erythematosus , Costimulation , dog