Record number :
1987105
Title of article :
Effects of GnRHa active immunity on FSHR expression and uterine development in prepuberty and non cyclic ewes
Author/Authors :
وِي، سواُچنگ نويسنده The Key Bio-Engineering and Technology Laboratory, National Nationality Commission, Northwest University For Nationalities, Lanzhou, Gansu Province, 730030, China Wei, S , گنگ، ژوآندي نويسنده Department of Gynecology, Medicine College, Northwest University for Nationalities, Lanzhou, Gansu Province, 730030, China Gong, Z , دونگ، جيانگ‌لينگ نويسنده Department of Reproduction Medicine, Life Science and Engineering College, Northwest University for Nationalities, Lanzhou, Gansu Province, 730030, China Dong, J , اويانگ، زياهو نويسنده Department of Reproduction Medicine, Life Science and Engineering College, Northwest University for Nationalities, Lanzhou, Gansu Province, 730030, China Ouyang, X , زي، كون نويسنده Department of Histology, Basic Medicine College, Lanzhou University, Lanzhou, Gansu Province, 730030, China Xie, K , وِي، مين نويسنده Department of Reproduction Medicine, Life Science and Engineering College, Northwest University for Nationalities, Lanzhou, Gansu Province, 730030, China Wei, M
Issue Information :
فصلنامه با شماره پیاپی 48 سال 2014
Pages :
6
From page :
244
To page :
249
Abstract :
اين مطالعه اثرات ايمني آگونيست GnRH بر روي بيان mRNA مربوط به FSHR در هيپوفيز و پروتئين FSRH در رحم را مورد بررسي قرار داد، و همچنين اثر بخشي آن بر روي تکامل رحم را تأييد نمود. 42 ميش غير سيکليک و نابالغ به شش گروه تجربي (EG) در خلال فصل استروس تخصيص داده شدند. حيوانات در EG-I، EG-II و EG-III به طور زير جلدي با 200، 300 و 400 ميکروگرم آنتي ژن آلارلين (روز 0 و 14) مورد تزريق قرار گرفتند. به حيوانات در EG-IV و EG-V به طور زير جلدي 200 و 300 ميکروگرم آنتي ژن آلارلين (روز 0، 7، 14 و 21) تزريق شد. بيان پروتئين FSHR رحمي با استفاده از وسترن بلاتينگ مشخص شد. RT-PCR براي اندازه‌گيري بيان mRNA مربوط به FSHR در روز 70 بعد از ايمني انجام شد. در مقايسه با CG، سطوح mRNA مربوط به FSHR هيپوفيز در EG-I، EG-II و EG-III تا 58%، 88% و 91% کاهش يافتند (P<0.01). در مقايسه با EG-I و EG-II mRNA مربوط به FSHR در EG-IV و EG-V تا 29/64% و 11/10% کاهش يافت (P<0.05). سطوح بيان پروتئين FSHR رحمي در EG-III و EG-V بيشتر از آن در CG بود. غدد رحمي، ميتوکندري‌ها و کريستاهاي ميتوکندريايي کم شدند، و حفره غده‌اي کاهش يافت، همچنين ميکروويلي‌ها کوتاه شدند. وزن شاخ‌هاي رحمي در EG-I، EG-II و EG-III به ترتيب تا 66/4%، 20/10% و 63/16% کاهش يافت. ضخامت ديواره رحمي (UWT) در EG-I، EG-II و EG-III تا 41/1%، 84/5% و 75/8% (P<0.05) کاهش يافت. ضخامت اپي‌تليوم اندومتر (EET) در EG-I، EG-II و EG-III به 70/2%، 87/8% و 15/19% کاهش يافت. روابط مثبت ميان وزن شاخ‌هاي رحمي و UWT، EET و FSHR محاسبه شدند.
Abstract :
This study explored the effects of GnRH agonist (GnRHa) immunity on expression of FSHR mRNA in pituitary and FSHR protein in uteri, and also confirmed its efficacy on uterine development. 42 prepuberty and non cyclic ewes were assigned to 6 experimental groups (EG) during anestrus season. Animals in EG-I, EG-II and EG-III were subcutaneously injected with 200, 300 and 400 μg alarelin antigens (day 0 and 14). Animals in EG-IV and EG-V were subcutaneously injected with 200 and 300 μg alarelin antigen (day 0, 7, 14 and 21). Uterine FSHR protein expression was detected using Western-blotting. RT-PCR was implemented to measure expression of pituitary FSHR mRNA on day 70 after the immunity. Compared to the CG, pituitary FSHR mRNA levels reduced by 58%, 88% and 91% (P<0.01) in EG-I, EG-II and EG-III. FSHR mRNA in EG-IV and EG-V reduced by 64.29% (P<0.05) and 10.11% compared to EG-I and EG-II. Expression levels of uterine FSHR protein in EG-III and EG-V were higher than that in CG. The uterine glands, mitochondria and mitochondrial crista decreased, and the glandular cavity reduced, also the microvilli shortened. Uterine horn weights in EG-I, EG-II and EG-III reduced by 4.66%, 10.20% and 16.63%, respectively. Uterine wall thickness (UWT) in EG-I, EG-II and EG-III reduced by 1.41%, 5.84% and 8.75% (P<0.05). The endometrial epithelium thickness (EET) in EG-I, EG-II and EG-III decreased 2.70%, 8.87% and 19.15%. The positive correlations between uterine horn weights and UWT, EET and FSHR were calculated.
Journal title :
Iranian Journal of Veterinary Research (IJVR)
Journal title :
Iranian Journal of Veterinary Research (IJVR)
Serial Year :
2014
Link To Document :
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