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Title of article :
Optimized extraction, separation and quantification of twelve intact glucosinolates in broccoli leaves
Author/Authors :
Ares، نويسنده , , Ana M. and Nozal، نويسنده , , Marيa J. and Bernal، نويسنده , , José L. and Bernal، نويسنده , , José، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
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Abstract :
A new method has been developed and validated to determine twelve intact glucosinolates (glucoiberin, GIB; glucoraphanin, GRA; glucoerucin GER; gluconapin, GNA; glucotropaeolin, GTL; glucobrassicin, GBC; gluconasturtiin, GST; glucoalyssin, ALY; 4-hydroxyglucobrassicin, 4-OH; 4-metoxyglucobrassicin, 4ME; neoglucobrassicin, NEO; sinigrin, SIN) in broccoli leaves using liquid chromatography (LC) coupled to diode array (DAD) and electrospray ionization mass spectrometry (ESI-MS) detection. An extraction procedure has also been proposed and optimized by means of statistical analysis (the Box–Behnken design and analysis of variance); this is based on the deactivation of myrosinase using a microwave and heated water. Low limits of detection and quantification were obtained, ranging from 10 to 72 μg/g with DAD and 0.01 to 0.23 μg/g with ESI-MS, and the resulting recovery values ranged from 87% to 106% in all cases. Finally, glucosinolates were analyzed in broccoli leaf samples from six different cultivars (Ramoso calabrese Parthenon, Marathon, Nubia, Naxos and Viola).
Keywords :
Broccoli leaves , LC-DAD–ESI-MS , Microwave extraction , Box–Behnken design , glucosinolates
Journal title :
Food Chemistry
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