Record number :
1965440
Title of article :
Development of a single-step precipitation cleanup method for the determination of enrofloxacin, ciprofloxacin, and danofloxacin in porcine plasma
Author/Authors :
Choi، نويسنده , , Jeong-Heui and Mamun، نويسنده , , M.I.R. and Abd El-Aty، نويسنده , , Am and Park، نويسنده , , Jong-Hyouk and Shin، نويسنده , , Eun-Ho and Yeon Park، نويسنده , , Ji and Cho، نويسنده , , Soon-Kil and Chul Shin، نويسنده , , Sung and Bong Lee، نويسنده , , Kang and Shim، نويسنده , , Jae-Han، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
6
From page :
1878
To page :
1883
Abstract :
In this study, we describe a newly developed and simple analytical method using high-performance liquid chromatography coupled to fluorescence detector (HPLC-FLD) for the determination of enrofloxacin, ciprofloxacin, and danofloxacin in porcine plasma. A single-step sample preparation, including extraction with acidic acetonitrile, coagulation with ammonium acetate, and centrifugation made possible the direct analysis of plasma samples without the need for any further cleanup procedure. The developed method was validated with specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, and precision. All results were fully adequate. In the study using porcine plasma incurring enrofloxacin, the developed method proved capable of quantifying concentrations below its maximum residue limit, and its time-course residues were excreted within the 10-day withdrawal time.
Keywords :
Porcine plasma , HPLC-FLD , Precipitation cleanup , Activated charcoal , withdrawal time , Fluoroquinolone
Journal title :
Food Chemistry
Journal title :
Food Chemistry
Serial Year :
2011
Link To Document :
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