Record number :
1965118
Title of article :
Fluorometric investigation on the interaction of oleanolic acid with bovine serum albumin
Author/Authors :
Cheng، نويسنده , , Zhengjun and Zhang، نويسنده , , Yuntao، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
7
From page :
81
To page :
87
Abstract :
The interactions between oleanolic acid and bovine serum albumin (BSA) have been studied by fluorescence, circular dichroism (CD), UV–vis absorption and Fourier transform infrared spectroscopy (FTIR) under physiological conditions. Spectroscopic analysis of the emission quenching at different temperatures has revealed that the quenching mechanism of bovine serum albumin by oleanolic acid is static quenching mechanism. The binding sites number n and binding constants K are obtained at various temperatures. The distance r between oleanolic acid and the protein is evaluated according to the theory of Forster energy transfer. The results by FTIR, CD and UV–vis absorption spectra experiment indicate that the secondary structures of protein have been perturbed in the presence of oleanolic acid. The thermodynamic parameters ΔH0, ΔG0, and ΔS0 are calculated according to van’t Hoff equation, which indicates that the hydrogen bonds and van der-waals are the intermolecular forces stabilizing the complex. Molecular modeling studies the interaction BSA with oleanolic acid.
Keywords :
Oleanolic acid , Fluorescence spectroscopy , circular dichroism spectroscopy , Bovine serum albumin , Fourier transform infrared spectroscopy
Journal title :
Journal of Molecular Structure
Journal title :
Journal of Molecular Structure
Serial Year :
2008
Link To Document :
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