Record number :
Title of article :
Antioxidant and free radical scavenging potential of Achillea santolina extracts
Author/Authors :
Ardestani، نويسنده , , Amin and Yazdanparast، نويسنده , , Razieh، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Pages :
From page :
To page :
Abstract :
The antioxidative activities of hydroalcoholic extract of Achillea santolina were investigated employing various established in vitro systems including total antioxidant activity in linoleic acid emulsion system, 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide and hydroxyl radicals scavenging, reducing power, and inhibitory effect on protein oxidation as well as the inhibition of Fe2+/ascorbate induced lipid peroxidation in rat liver homogenate. Total phenolic and flavonoid content of A. santolina extract (ASE) was also determined by a colorimetric method. The results revealed that ASE has notable inhibitory activity on peroxides formation in linoleic acid emulsion system along with concentration-dependent quenching of DPPH and superoxide radicals. Furthermore, the extract showed both nonsite-specific (Fe2+ + H2O2 + EDTA) and site-specific (Fe2+ + H2O2) hydroxyl radical scavenging suggesting potent hydroxyl radical scavenging and chelating ability for iron ions in deoxyribose degradation model. A linear correlation between ASE and the reducing power was also observed (r2 = 0.9981). ASE prevents thiobarbituric acid reactive substances formation in Fe2+/ascorbate induced lipid peroxidation in rat liver tissue in a dose-dependent manner. Moreover, free radical induced protein oxidation was suppressed significantly by the addition of ASE over a range of concentration. These results clearly demonstrated that A. santolina extract possess a marked antioxidant activity.
Keywords :
Achillea santolina , Reactive oxygen species , antioxidant , Lipid peroxidation
Journal title :
Food Chemistry
Serial Year :
Link To Document :