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Title of article :
Metabolically engineered glucose-utilizing Shewanella strains under anaerobic conditions
Author/Authors :
Choi، نويسنده , , Donggeon and Lee، نويسنده , , Sae-Bom and Kim، نويسنده , , Sohyun and Min، نويسنده , , Byoungnam and Choi، نويسنده , , In-Geol and Chang، نويسنده , , In Seop، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
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Abstract :
Comparative genome analysis of Shewanella strains predicted that the strains metabolize preferably two- and three-carbon carbohydrates as carbon/electron source because many Shewanella genomes are deficient of the key enzymes in glycolysis (e.g., glucokinase). In addition, all Shewanella genomes are known to have only one set of genes associated with the phosphotransferase system required to uptake sugars. To engineer Shewanella strains that can utilize five- and six-carbon carbohydrates, we constructed glucose-utilizing Shewanella oneidensis MR-1 by introducing the glucose facilitator (glf; ZMO0366) and glucokinase (glk; ZMO0369) genes of Zymomonas mobilis. The engineered MR-1 strain was able to grow on glucose as a sole carbon/electron source under anaerobic conditions. The glucose affinity (Ks) and glucokinase activity in the engineered MR-1 strain were 299.46 mM and 0.259 ± 0.034 U/g proteins. The engineered strain was successfully applied to a microbial fuel cell system and exhibited current generation using glucose as the electron source.
Keywords :
Shewanella oneidensis MR-1 , anaerobic conditions , Glucose Utilization , Current generation , Microbial fuel cells
Journal title :
Bioresource Technology
Journal title :
Bioresource Technology
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