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Title of article :
Expression of multi-functional cellulase gene mfc in Coprinus cinereus under control of different basidiomycete promoters
Author/Authors :
Cheng، نويسنده , , Shujie and Yang، نويسنده , , Peizhou and Guo، نويسنده , , Liqiong and Lin، نويسنده , , Junfang and Lou، نويسنده , , Nannan، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
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Abstract :
Multi-functional cellulase gene mfc was expressed in Coprinus cinereus under naturally non-inductive conditions using three heterologous promoters. Endo-β-1,4-glucanase expression was achieved in solid and liquid media with promoter sequences from the Lentinula edodes gpd gene, the Flammulina velutipes gpd gene and the Volvariella volvacea gpd gene. As measured by enzyme activity in liquid cultures, a 613-bp gpd promoter fragment from L. edodes was most efficient, followed by a 752-bp gpd fragment from F. velutipes. The V. volvacea gpd promoter sequence was less active, in comparison. Irrespective of the promoter used, enzymatic activities increase 34-fold for highly active transformants and 29-fold for less active one by using cellulase-inducing medium. The highest activities of endo-β-1,4-glucanase (34.234 U/ml) and endo-β-1,4-xylanase (263.695 U/ml) were reached by using the L. edodes gpd promoter.
Keywords :
4-xylanase , 4-glucanase , Coprinus cinereus , Basidiomycete promoters , Multi-functional cellulase , endo-?-1 , endo-?-1
Journal title :
Bioresource Technology
Journal title :
Bioresource Technology
Serial Year :
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