Record number :
Title of article :
Altering n-3 to n-6 polyunsaturated fatty acid ratios affects prostaglandin production by ovine uterine endometrium
Author/Authors :
Cheng، نويسنده , , Zhangrui and Abayasekara، نويسنده , , D. Robert E. and Ward، نويسنده , , Freya and Preece، نويسنده , , Daniel M.W. and Raheem، نويسنده , , Kabir A. and Wathes، نويسنده , , D. Claire، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
From page :
To page :
Abstract :
Consumption of n-3 polyunsaturated fatty acids (PUFAs) is considered beneficial to health but effects on fertility remain uncertain. This study investigated the effect of n-3 PUFA supplementation on endometrial prostaglandin (PG) production. Ovine uterine endometrial cells were cultured to confluence in DMEM/F12 medium containing 10% foetal bovine serum. Stromal and epithelial cell populations were confirmed by immunocytochemistry. Cultures were supplemented with 0, 20 or 100 μM of α-linolenic acid (ALA), stearidonic acid (SDA), eicosapentaenoic acid (EPA) with lipopolysaccharide (LPS) at 0 and 0.1 μg/ml, or different combinations of EPA with arachidonic acid (AA) in serum-free medium for 24 h. PGs were quantified using radioimmunoassay and PG-endoperoxide synthase (PTGS) isoforms, PGE and PGF synthase (microsomal PGES1 and PGFS) mRNAs by qPCR. LPS increased PGE2 production significantly without changing PGF2α production, causing increased PGE2:PGF2α ratios. ALA and SDA increased PGE2, PGF2α and PGE2:PGF2α ratios (P < 0.05–0.01) while EPA alone did not affect PG generation. AA significantly stimulated PTGS1 and PTGS2 mRNA expression and PGE2 and PGF2α production (P < 0.01). The stimulatory effect of AA was attenuated by up to 80% (P < 0.05) when AA was combined with EPA. The PGE2:PGF2α ratio was not affected by AA or EPA alone, but increased when these two PUFAs were combined (P < 0.05). SDA and EPA decreased PTGS1 mRNA expression (P < 0.05) but did not alter PTGS2 expression. EPA and AA up-regulated mPGES1 expression (P < 0.05) without affecting PGFS expression. Since AA is preferentially incorporated in uterine endometrium to produce 2-series PGs, alteration of PG production by EPA may affect many reproductive processes.
Keywords :
Polyunsaturated fatty acid , Endometrial cells , Ewe , prostaglandins , Uterus , Prostaglandin-endoperoxide synthase
Journal title :
Animal Reproduction Science
Journal title :
Animal Reproduction Science
Serial Year :
Link To Document :