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Title of article :
Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
Author/Authors :
Rodrيguez، نويسنده , , F.M. and Salvetti، نويسنده , , N.R. and Colombero، نويسنده , , M. and Stangaferro، نويسنده , , M.L. and Barbeito، نويسنده , , C.G. and Ortega، نويسنده , , H.H. and Rey، نويسنده , , F.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
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Abstract :
Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P < 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
Keywords :
Ovaries , cattle , Cystic ovarian disease , Insulin-Like Growth Factor
Journal title :
Animal Reproduction Science
Link To Document :