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Title of article :
LASP-1, a Novel Urinary Marker for Detection of Bladder Cancer
Author/Authors :
Ardelt، نويسنده , , Peter and Grünemay، نويسنده , , Nadine and Strehl، نويسنده , , Annette and Jilg، نويسنده , , Cordula and Miernik، نويسنده , , Arkadius and Kneitz، نويسنده , , Burkhard and Butt، نويسنده , , Elke، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
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Abstract :
Objectives M and SH3 (LASP)-1 protein is a focal adhesion protein that has been linked to oncogenesis. The aim of this study was to evaluate the diagnostic use of the detection of LASP-1 in tumor specimens and in urine for noninvasive detection of transitional cell carcinoma (TCC). als and methods histochemical staining for LASP-1 was performed on 72 archived bladder tumor specimens, and LASP-1 content was measured in 132 spontaneous urine sample sediments by Western blot analysis. s histologic specimens, immunohistochemical staining for LASP-1 showed abundant expression throughout the urothelium of the bladder and ureter. However, modest overexpression of LASP-1 was observed in the TCC specimens. Measurement of the LASP-1 protein concentrations in urinary cell pellets from healthy donors and bladder cancer patients was highly sensitive for the presence of TCC. The cut-off value was determined by receiver operating characteristic (ROC) analysis. When a cut-off value of 1 ng LASP-1/500 μl of urine was used, the sensitivity, specificity, and positive and negative predictive values of the assay were 83.1%, 85.3%, 83.1%, and 80.6%, respectively.The increased urinary LASP-1 content in TCC patients was attributable in part to a specific increase in cell shedding presumably caused by changes in cell adhesion, as confirmed by LASP-1 knockdown. Contamination with erythrocytes above 250 cells/μl and urinary infection gave false positive results and are therefore sample exclusion criteria. sions absence of urinary infection or gross hematuria, urinary LASP-1 level is a promising marker for transitional cell carcinoma.
Keywords :
Transitional cell carcinoma , LASP-1 , bladder cancer
Journal title :
Urologic Oncology
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