Record number :
Title of article :
UV radiation effects on flavocytochrome b2 in dilute aqueous solution
Author/Authors :
Bhattacharya، نويسنده , , Debjani and Basu، نويسنده , , Samita and Mandal، نويسنده , , Parikshit C.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
Pages :
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Abstract :
A dilute aqueous solution of flavocytochrome b2 when exposed to inactivating doses of UV radiation at 280 nm underwent reversible loss in activity both under aerated and deaerated conditions. The active site as well as the substrate binding sites were found to be modified in the irradiated enzyme. Irradiation of the enzyme in the UV-C range resulted in partial unfolding of the polypeptide framework. Destruction and/or modification of both tryptophan and tyrosine residues as well as heme moieties took place. Preliminary laser flash photolysis studies suggest that the initial photo-ionization takes place with tryptophan and tyrosine residues with the formation of excited states and radicals, and then rapid transfer of electrons takes place to histidyl and cystinyl sites which might have eventually been altered in the process.
Keywords :
tyrosine , Heme , Radical formation and enzyme inactivation , Tryptophan
Journal title :
Journal of Photochemistry and Photobiology B:Biology
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Link To Document :