Record number :
1855104
Title of article :
Human T cell development in the liver of humanized NOD/SCID/IL-2Rγnull(NSG) mice generated by intrahepatic injection of CD34+ human (h) cord blood (CB) cells
Author/Authors :
Choi، نويسنده , , Bongkum and Chun، نويسنده , , Eunyoung and Kim، نويسنده , , Miyoung and Kim، نويسنده , , So Yong and Kim، نويسنده , , Seong-Tae and Yoon، نويسنده , , Keejung and Lee، نويسنده , , Ki-Young and Kim، نويسنده , , Sung Joo، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
15
From page :
321
To page :
335
Abstract :
In this study, we explore the possibility of human T cell development in the liver of humanized mice generated by intrahepatic injection of CD34+ hCB cells into conditioned NOD/SCID/IL-2Rγnull(NSG) newborn mice. The intrahepatic injection of CD34+ hCB cells led to effective reconstitution of human myeloid and lymphoid lineage cells. In contrast to the previously reported Rag2−/−γc−/− humanized mice, interestingly, the thymus function of humanized NSG mice was markedly reduced in terms of its size and cell contents, whereas the livers of humanized NSG mice profoundly contained double-positive (DP), hCD4 and hCD8 single positive (SP), hCD34+hCD38lohCD1a− (TSP), hCD34+hCD38hihCD1a− (ETP), and hCD34+hCD38+hCD1a+ (pre-T cells) cells. Furthermore, immunostaining of the liver revealed that human T cells were co-localized with hDCs. Taken together, our results demonstrate that the intrahepatic injection of hCD34+ hCB cells can facilitate human T cell development in the livers of humanized NSG mice.
Keywords :
Humanized NSG mice , CD34+ human CB cells , Intrahepatic injection , Human T cell development
Journal title :
Clinical Immunology
Journal title :
Clinical Immunology
Serial Year :
2011
Link To Document :
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