Record number :
Title of article :
Aberrant methylation of ADAMTS1 in non-small cell lung cancer
Author/Authors :
Choi، نويسنده , , Jin Eun and Kim، نويسنده , , Dong Sun and Kim، نويسنده , , Eun Jin and Chae، نويسنده , , Myung Hwa and Cha، نويسنده , , Sung Ick and Kim، نويسنده , , Chang Ho and Jheon، نويسنده , , Sanghoon and Jung، نويسنده , , Tae Hoon and Park، نويسنده , , Jae Yong، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
From page :
To page :
Abstract :
ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin type 1 motifs) is an extracellular matrix metalloproteinase with protease activity and antiangiogenic activity. It has been suggested that ADAMTS1 plays an important role in tumor growth and metastasis. In this study, we examined ADAMTS1 expression in non-small cell lung cancer (NSCLC), and we also evaluated whether the loss of ADAMTS1 expression is due to aberrant methylation of the gene. In addition, we examined the relationship between ADAMTS1 methylation and clinicopathologic features in NSCLC patients. ADAMTS1 expression was examined using reverse-transcription polymerase chain reaction (PCR), and the methylation status of the gene was evaluated by methylation-specific PCR in NSCLC cell lines (n=10) and primary NSCLC tumors (n=98). Down-regulation of ADAMTS1 was observed in 30% (3/10) of the NSCLC cell lines, and this down-regulation was found to be concordant with aberrant methylation of the gene. Furthermore, ADAMTS1 expression was restored after treatment with the demethylating agent, 5-Aza-2’-deoxycytidine, in cell lines that lacked ADAMTS1 expression. Aberrant methylation of the gene was observed in 31.6% (31 of 98) of the NSCLC tumors, while it was found in only 7.1% (7/98) of the corresponding nonmalignant tissues. Methylation in NSCLC tumors was not correlated with the clinicopathologic features of the patients, such as age, gender, and histology and pathologic staging of the tumor. Taken together, these results suggest that aberrant methylation of ADAMTS1 frequently occurs in NSCLCs and that it may play a role in the pathogenesis of NSCLC.
Journal title :
Cancer Genetics and Cytogenetics
Journal title :
Cancer Genetics and Cytogenetics
Serial Year :
Link To Document :