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Title of article :
NEMO-binding domain peptide inhibits proliferation of human melanoma cells
Author/Authors :
Angela Ianaro، نويسنده , , Angela and Tersigni، نويسنده , , Mariaroberta and Belardo، نويسنده , , Giuseppe De Martino، نويسنده , , Silvana Di and Napolitano، نويسنده , , Maria and Palmieri، نويسنده , , Giuseppe and Sini، نويسنده , , MariaCristina and Maio، نويسنده , , Anna De and Ombra، نويسنده , , MariaNeve and Gentilcore، نويسنده , , Giuseppina and Capone، نويسنده , , Mariaelena and Ascierto، نويسنده , , MariaLibera and Satriano، نويسنده , , Rocco Alfredo and Farina، نويسنده , , Benedetta and Faraone-Mennella، نويسنده , , MariaRosaria and Ascierto، نويسنده , , Paolo Antonio and Ialenti، نويسنده , , Armando، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
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Abstract :
Melanoma is the most aggressive form of skin cancer, it originates from melanocytes and its incidence has increased in the last decade. Recent advances in the understanding of the underlying biology of the progression of melanoma have identified key signalling pathways that are important in promoting melanoma tumourigenesis, thus providing dynamic targets for therapy. One such important target identified in melanoma tumour progression is the Nuclear Factor-κB (NF-κB) pathway. In vitro studies have shown that NF-κB binding is constitutively elevated in human melanoma cultures compared to normal melanocytes. It has been found that a short cell-permeable peptide spanning the IKK-β NBD, named NBD peptide, disrupted the association of NEMO with IKKs in vitro and blocked TNFα-induced NF-κB activation in vivo. In the present study we investigated the effect of the NBD peptide on NF-κB activity and survival of A375 human melanoma cells. We found that NBD peptide is able to inhibit the proliferation of A375 cells, which present constitutively elevated NF-κB levels. Inhibition of cell proliferation by NBD peptide was associated with direct inhibition of constitutive NF-κB DNA-binding activity and induction of apoptosis by activation of caspase-3 as confirmed by the cleavage and consequently inactivation of poly (ADP ribose) polymerase (PARP-1) known as the best marker of this process.
Keywords :
NBD peptide , NF-?B , apoptosis , melanoma
Journal title :
Cancer Letters
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Link To Document :