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Title of article :
Tumor-conditioned medium increases macromolecular permeability of endothelial cell monolayer
Author/Authors :
Utoguchi، نويسنده , , Naoki and Mizuguchi، نويسنده , , Hiroyuki and Saeki، نويسنده , , Kazuhiko and Ikeda، نويسنده , , Kenji and Tsutsumi، نويسنده , , Ysauo and Nakagawa، نويسنده , , Shinsaku and Mayumi، نويسنده , , Tadanori، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1995
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Abstract :
The permeation of macromolecular FITC-labeled dextran (molecular weight 70 000) through bovine aortic endothelial cells (BAEC) monolayer, which were cultured for 5 days with conditioned medium prepared from mouse melanoma B16, was increased. However, when BAEC, which were cultured with normal medium until confluent, were treated with B16 conditioned medium (B16-CM) for 30 min, the permeability did not increase. The B16-CM also increased the permeability of the endothelial monolayers of bovine veins and the human umbilical vein, but did not increase that of the epithelial monolayer. The B16-CM did not alter the distribution or content of F-actin on the BAEC. BAEC cultured in the presence of B16-CM for 5 days were detached from the dish, and then seeded into a chamber at one-fifth of confluent cell density. After 5 days of culture in normal medium, the BAEC were grown to confluence and their permeability was increased. These findings suggest that B16-CM increased the endothelial permeability irreversibly without the decrease of F-actin, and that soluble factor(s) which were secreted from the tumor cells participate in the construction of the hyperpermeable structure of tumor vessels in vivo.
Keywords :
Tumor , F-actin , endothelial cell , Permeability , Conditioned medium
Journal title :
Cancer Letters
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