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Title of article :
Platelet-derived growth-factor-releasing aligned collagen–nanoparticle fibers promote the proliferation and tenogenic differentiation of adipose-derived stem cells
Author/Authors :
Cheng، نويسنده , , XingGuo and Tsao، نويسنده , , Christopher and Sylvia، نويسنده , , Victor L. and Cornet، نويسنده , , Douglas and Nicolella، نويسنده , , Daniel P. and Bredbenner، نويسنده , , Todd L. and Christy، نويسنده , , Robert J.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
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Abstract :
In order to enhance the healing potential of an injured tendon, we have prepared a novel biomimetic aligned collagen–nanoparticle (NP) composite fiber using an electrochemical process. The aligned collagen–NP composite fiber is designed to affect the cellular activity of adipose-derived stem cells (ADSCs) through two different ways: (i) topographic cues from the alignment of collagen fibril and (ii) controlled release of platelet-derived growth factors (PDGFs) from the NPs. PDGF released from collagen–NP fibers significantly enhanced the proliferation of ADSCs when tested for up to 7 days. Moreover, compared to random collagen fibers with PDGFs, aligned collagen–NP fibers significantly promoted the desirable tenogenic differentiation of ADSCs, as evidenced by an increased level of tendon markers such as tenomodulin and scleraxis. On the other hand, no undesirable osteogenic differentiation, as measured by the unchanged level of alkaline phosphatase and osteocalcin, was observed. Together, these results indicate that the aligned collagen–NP composite fiber induced the tenogenic differentiation of ADSCs through both a topographic cue (aligned collagen fibril) and a chemical cue (PDGF released from NPs). Thus, our novel aligned collagen–NP composite fiber has a significant potential to be used for tendon tissue engineering and regeneration.
Keywords :
stem cell , Tendon , Collagen , Nanoparticle , Growth factors
Journal title :
Acta Biomaterialia
Journal title :
Acta Biomaterialia
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Link To Document :