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Title of article :
Separation and determination of Se-compounds by liquid chromatography coupled with electrospray mass spectrometry
Author/Authors :
Carmen Barciela-Alonso، نويسنده , , M. and Bermejo-Barrera، نويسنده , , Adela and Bermejo-Barrera، نويسنده , , Pilar، نويسنده ,
Issue Information :
فصلنامه با شماره پیاپی سال 2007
Pages :
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Abstract :
A method for Selenocystine and Selenomethionine determination by LC–ES–MS was developed in this work. The mass spectrometer was used in a positive mode and the m/z used for the identification of Selenomethionine and Selenocystine were 198.35 and 337.15, respectively. lenium species were separated using a LC system. A silica chromatographic column (ZORBAX Eclipse XDB-C8 of 50 mm length and 2.1 mm internal diameter (particle size 3.5 μm)) was used. The separation was realised in isocratic mode, using methanol:water (1:1) with 1% of acetic acid and a flow rate of 200 μL min−1. The developed method was precise (RSD of 4.5% and 3.9% for Selenomethionine and Selenocystine, respectively) and sensible (limit of detection (LOD) 0.06 and 0.99 mg L−1 for selenomethionine and selenocystine, respectively).
Keywords :
Seleno-compounds , Liquid chromatography , mass spectrometry
Journal title :
Journal of Trace Elements in Medicine and Biology
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