Record number :
1706011
Title of article :
Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen–phosphorus detection
Author/Authors :
Choi، نويسنده , , Hak Soo and Shin، نويسنده , , Ho-Chul and Khang، نويسنده , , Gilson and Rhee، نويسنده , , John M and Lee، نويسنده , , Hai Bang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Pages :
7
From page :
63
To page :
69
Abstract :
A sensitive assay method was developed to determine fentanyl, an opiate agonist, in rat plasma by gas chromatography with nitrogen–phosphorus detection. For the pretreatment of plasma samples, sodium hydroxide was added to denature protein and n-butyl chloride was used to extract fentanyl. The calibration curve was linear within the concentration range 0.5 to 50 ng/ml (r=0.9997). The limit of detection was 0.1 ng/ml, and 0.5 ng/ml could be quantified with acceptable precision. Furthermore, fentanyl could be determined in only 200 μl of rat plasma. The method has been successfully applied to an intramuscular pharmacokinetic study at a dose of 10 μg/kg. Therefore, the current method is a valuable analytical tool for investigating the pharmacokinetics of fentanyl at low clinical doses.
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications
Serial Year :
2001
Link To Document :
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