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Title of article :
Determination of urine catecholamines by capillary electrophoresis with dual-electrode amperometric detection
Author/Authors :
Chen، نويسنده , , Der-chang and Zhan، نويسنده , , Dian-Zhen and Cheng، نويسنده , , Chun-Weng and Liu، نويسنده , , An-Chiang and Chen، نويسنده , , Chun-hsien، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
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Abstract :
Demonstrated in this study is that without pretreatment and preconcentration nanomolar-level catecholamines in human urine samples can be quantitatively determined with ease by utilizing capillary electrophoresis coupled with amperometric detection. The detector employs a parallel-opposed dual-electrode scheme assembled with an on-capillary electrode and a disk electrode and takes advantage of the redox cycling of analytes between the two working electrodes to improve the limit of detection. The matrix effect of urine samples significantly decreases the detection sensitivity from that obtained in standard solutions. Therefore, calibration curves derived from standard solutions cannot be used in quantitative determination of catecholamines. Methods of standard addition and internal standard have been studied. The results suggest that isoproterenol is a good internal standard to facilitate the measurements of dopamine, epinephrine, and norepinephrine in human urine samples.
Keywords :
Dopamine , Norepinephrine , catecholamines , Epinephrine
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications
Link To Document :