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Title of article :
Liquid chromatographic–mass spectrometric determination of itraconazole and its major metabolite, hydroxyitraconazole, in dog plasma
Author/Authors :
Carrier، نويسنده , , Alain and Parent، نويسنده , , Josée، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
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Abstract :
A fast, reliable and sensitive liquid chromatography–mass spectrometry (LC–MS) assay for the determination of itraconazole and hydroxyitraconazole in dog plasma has been developed. The analysis involves a simple liquid–liquid extraction followed by LC–MS analysis using electrospray ionization in the positive mode. Total separation of itraconazole, hydroxyitraconazole and the internal standard, miconazole, was achieved on a C18 column in 3.5 min using an isocratic mixture of acetonitrile and 10 mM ammonium acetate. The response was linear over four-orders of magnitude, allowing reliable quantification of each species. This paper describes the development of the method and its validation.
Keywords :
Itraconazole , Hydroxyitraconazole
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications
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