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Title of article :
Development of a high-performance liquid chromatographic–mass spectrometric assay for the specific and sensitive quantification of Ro 64-0802, an anti-influenza drug, and its pro-drug, oseltamivir, in human and animal plasma and urine
Author/Authors :
Wiltshire، نويسنده , , Hugh and Wiltshire، نويسنده , , Barbara and Citron، نويسنده , , Andrea and Clarke، نويسنده , , Tony and Serpe، نويسنده , , Carolyn M. Gray، نويسنده , , Donald and Herron، نويسنده , , William، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
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Abstract :
Oseltamivir phosphate (Ro 64-0796/002) is a pro-drug of the anti-influenza neuraminidase inhibitor, Ro 64-0802, and as Tamiflu™, has been developed for the treatment of both A and B strains of the disease. This paper describes an HPLC–MS–MS assay for both compounds in plasma and urine which fulfils all of the criteria for a good analytical method. It is sensitive with limits of quantification of 1 and 10 ng/ml for the pro-drug and active neuraminidase inhibitor, respectively. It is both accurate and precise with typical coefficients of variation from some 5000 quality control samples of approximately ±3 and ±6%, respectively. Extensive stability studies have demonstrated the absence of significant problems associated with the decomposition of either compound, although ex vivo hydrolysis of Ro 64-0796 to Ro 64-0802 in rodent plasma has to be prevented by the use of the esterase inhibitor, dichlorvos.
Keywords :
Oseltamivir , Ro 64-0802
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications
Link To Document :