Record number :
1663014
Title of article :
Relationships between PSII-independent hydrogen bioproduction and starch metabolism as evidenced from isolation of starch catabolism mutants in the green alga Chlamydomonas reinhardtii
Author/Authors :
Chochois، نويسنده , , Vincent and Constans، نويسنده , , Laure and Dauvillée، نويسنده , , David and Beyly، نويسنده , , Audrey and Solivérès، نويسنده , , Mélanie and Ball، نويسنده , , Steven and Peltier، نويسنده , , Gilles and Cournac، نويسنده , , Laurent، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
10
From page :
10731
To page :
10740
Abstract :
Sulfur deprivation, which is considered as an efficient way to trigger long-term hydrogen photoproduction in unicellular green algae has two major effects: a decrease in PSII which allows anaerobiosis to be reached and carbohydrate (starch) storage. Starch metabolism has been proposed as one of the major factors of hydrogen production, particularly during the PSII-independent (or indirect) pathway. While starch biosynthesis has been characterized in the green alga Chlamydomonas reinhardtii, little remains known concerning starch degradation. In order to gain a better understanding of starch catabolism pathways and identify those steps likely to limit the starch-dependent hydrogen production, we have designed a genetic screening procedure aimed at isolating mutants of the green alga C. reinhardtii affected in starch mobilization. Using two different screening protocols, the first one based on aerobic starch degradation in the dark and the second one on anaerobic starch degradation in the light, eighteen mutants were isolated among a library of 15,000 insertion mutants, eight (std1-8) with the first screen and ten (sda1-10) with the second. Most of the mutant strains isolated in this study showed a reduction or a delay in the PSII-independent hydrogen production. Further characterization of these mutants should allow the identification of molecular determinants of starch-dependent hydrogen production and supply targets for future biotechnological improvements.
Keywords :
biohydrogen , Chlamydomonas , Hydrogen production , Starch mutant , Starch catabolism , Sulfur deficiency
Journal title :
International Journal of Hydrogen Energy
Journal title :
International Journal of Hydrogen Energy
Serial Year :
2010
Link To Document :
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