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Title of article :
Coelectroosmotic capillary electrophoresis of phenolic acids and derivatized amino acids using N,N-dimethylacrylamide-ethylpyrrolidine methacrylate physically coated capillaries
Author/Authors :
Carrasco-Pancorbo، نويسنده , , Alegrيa and Cifuentes، نويسنده , , Alejandro and Cortacero-Ramيrez، نويسنده , , Sonia and Segura-Carretero، نويسنده , , Antonio and Fernلndez-Gutiérrez، نويسنده , , Alberto، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2007
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Abstract :
Two different families of compounds, i.e., phenolic and amino acids have been separated by capillary electrophoresis using a physically adsorbed polymer as capillary coating. The polymer used was N,N-dimethylacrylamide-ethylpyrrolidine methacrylate (DMA-EpyM) and it provided an stable coating by only flushing the capillary with a DMA-EpyM aqueous solution for 2 min between runs. The usefulness of this procedure has been demonstrated through the fast analysis of different families of solutes. Two different detection systems, diode-array detector and laser-induced fluorescence, have been used to determine phenolic acids and derivatized amino acids with fluorescein isothiocyanate, respectively. The main factors affecting reversal of electroosmotic flow (EOF) such as pH, type and concentration of buffer, and concentration and influence of organic solvents, as well as all the instrumental conditions were studied and optimized for both families of compounds.
Keywords :
Coelectroosmotic capillary electrophoresis , amino acids , Coating , laser-induced fluorescence , DMA-EpyM , Phenolic acids
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