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Title of article :
High production of cellulose degrading endo-1,4-β-d-glucanase using bagasse as a substrate from Bacillus subtilis KIBGE HAS
Author/Authors :
Bano، نويسنده , , Saeeda and Qader، نويسنده , , Shah Ali Ul and Aman، نويسنده , , Afsheen and Syed، نويسنده , , Mohammad Noman and Durrani، نويسنده , , Kamran، نويسنده ,
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روزنامه با شماره پیاپی سال 2013
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Abstract :
Sugarcane bagasse is a cheap carbon source for endo-1,4-β-d-glucanase production as it is easily available as by-product from sugar industries. Fermentation conditions for endo-1,4-β-d-glucanase production by Bacillus subtilis KIBGE HAS were optimized by using un-treated sugarcane bagasse for induction of endo-1,4-β-d-glucanase and it was found that 2.0 g% bagasse in fermentation medium induced maximum endo-1,4-β-d-glucanase production. It was also found that when sugarcane bagasse was supplemented with different carbon sources, the results showed that lactose, xylose, maltose and sucrose favored endo-1,4-β-d-glucanase production, whereas cellobiose and fructose inhibit enzyme production. Maximum endo-1,4-β-d-glucanase production was obtained at 40 °C keeping the initial pH of the medium at 7.0 before sterilization. Maximum endo-1,4-β-d-glucanase production was obtained after 48 h incubation. Among different nitrogen sources, ammonium nitrate enhanced endo-1,4-β-d-glucanase production. The optimal temperature and pH for enzyme activity were 60 °C and 7.0, respectively.
Keywords :
4-?-d-glucanase , endo-1 , sugarcane bagasse , BACILLUS SUBTILIS , Carbon source
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