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Title of article :
Chemical and biological characterization of pectin-like polysaccharides from the bark of the Malian medicinal tree Cola cordifolia
Author/Authors :
Austarheim، نويسنده , , Ingvild and Christensen، نويسنده , , Bjّrn E. and Hegna، نويسنده , , Ida K. and Petersen، نويسنده , , Bent O. and Duus، نويسنده , , Jens O. and Bye، نويسنده , , Ragnar and Michaelsen، نويسنده , , Terje E. and Diallo، نويسنده , , Drissa and Inngjerdingen، نويسنده , , Marit and Paulsen، نويسنده , , Berit S.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
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Abstract :
The bark of Cola cordifolia used in Malian traditional medicine contains unusual types of polysaccharides with immunomodulating activities. We report for the first time on the structure of a polymer designated CC1P1 having the repeating structure [2→)[α-d-Gal(1→3)]α-l-Rha(1→4)α-d-GalA(1→] as determined by NMR and GC/MS. α-Linked Gal is unusual in pectins. The Mw of 135 kDa was determined by SEC-MALLS. CC1P2 (1400 kDa), another polymer, having the same backbone, but this was substituted with α-4-OMe-GlcA, α-2-OMe-Gal and α-Gal as terminal units. CC1P1 shows a high complement-fixing activity, IC50 being 2.2 times lower than the positive pectin control PMII (IC50 appr. 71 μg/mL) while IC50 of CC1P2 is 1.8 times lower. The simple structure of CC1P1 did not activate macrophages, while CC1P2 (100 μg/mL) showed the same potency as the positive controls PMII (100 μg/mL) and LPS (500 ng/mL). No cytotoxicity was detected.
Keywords :
Immunological activity , cytotoxicity , Cola cordifolia , pectin , Complement system , Rhamnogalacturonan , structure
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