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Title of article :
Purification of a polysaccharide from Boschniakia rossica and its synergistic antitumor effect combined with 5-Fluorouracil
Author/Authors :
Wang، نويسنده , , Zhenghui and Wu، نويسنده , , Baojun and Zhang، نويسنده , , Xianghong and Xu، نويسنده , , Min and Chang، نويسنده , , Huimin and Lu، نويسنده , , Xiaoyun and Ren، نويسنده , , Xiaoyong، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
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Abstract :
Current study we purified a polysaccharide (BRP) from Boschniakia rossica and the antitumor effects of BRP alone or combined with 5-Fluorouracil (5-FU) was examined in S180 tumor bearing mice by intragastric administration. The high performance size-exclusion chromatography (HPGEC) analysis showed that BRP was a homogeneous polysaccharide and had a molecular weight of 2.2 × 104 Da. Total carbohydrate content in BRP was determined to be 96.9%, without the presence of protein and nucleic acid. BRP alone or combined with 5-FU could significantly inhibit Sarcoma-180 (S180) tumor growth and increase the spleen index in a dose-dependent manner. Meanwhile a synergistic effect was observed in boosting various immunity functions when the tumor bearing mice receiving BRP plus 5-FU administration, such as stimulating lymphocytes proliferation, increasing NK cell cytotoxicity, enhancing serum interleukin-2 (IL-2) and interferon-gamma (TNF-γ) secretion, as well as augmenting CD4+ and CD8+ spleen T lymphocytes subsets. The results showed that BRP combined with 5-FU presented synergistic effects on antitumor activity in tumor bearing mice. In conclusion, the combination of BRP may boost the suppressed immunity in tumor bearing mice subject to 5-FU chemotherapy, and could serve as a new, promising approach for cancer treatment.
Keywords :
polysaccharide , Immunomodulatory , Adjuvant , antitumor , Boschniakia rossica
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