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Title of article :
Structural characterization of pellicle polysaccharides of Acetobacter tropicalis SKU1100 wild type and mutant strains
Author/Authors :
Ali، نويسنده , , Ibnaof Ali Ibnaof and Akakabe، نويسنده , , Yoshihiko and Moonmangmee، نويسنده , , Somporn and Deeraksa، نويسنده , , Arpaporn and Matsutani، نويسنده , , Minenosuke and Yakushi، نويسنده , , Toshiharu and Yamada، نويسنده , , Mamoru and Matsushita، نويسنده , , Kazunobu، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
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Abstract :
Mutants of Acetobacter tropicalis SKU1100 (R) strain, ΔpolE and ΔgalE, defective in pellicle formation, excrete exopolysaccharide (EPS) instead of capsular polysaccharide (CPS) that is produced by the wild-type. We carried out structural analysis of wild type CPS and mutant EPSs using monosaccharide composition analysis, methylation analysis, and 1H and 13C NMR spectroscopy. Wild-type CPS and ΔpolE mutant EPS had a branched hexasaccharide repeating unit composed of 2 moles of 2,3-α-l-rhamnopyranosyl, and 1 mole each of 6-β-d-galactopyranosyl and 2-α-d-glucopyranosyl residues, of which the rhamnosyl residues were branched by terminal-β-d-galactofuranosyl and terminal-α-d-glucopyranosyl residues. The EPS of ΔgalE mutant showed a branched tetrasaccharide repeating unit composed of 2,3-α-l-rhamnopyranosyl, 2-α-d-glucopyranosyl, and 3-α-l-rhamnopyranosyl residues and terminal-α-d-glucopyranosyl branch residue. By comparing the three structures, it was suggested that PolE may control the switching of EPS to CPS by adding some residue, e.g. β-d-galactopyranosyl residue, to 2,3-α-l-rhamnopyranosyl residue to make 2,3,4-α-l-rhamnosyl residue which leads to CPS formation.
Keywords :
Pellicle , Acetobacter tropicalis , Structural analysis , polysaccharide
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