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Title of article :
Cloning and Expression of theglgCGene fromAgrobacterium tumefaciens:Purification and Characterization of the ADPglucose Synthetase
Author/Authors :
Uttaro، نويسنده , , Antonio D and Ugalde، نويسنده , , Rodolfo A and Preiss، نويسنده , , Jack and Iglesias، نويسنده , , Alberto A، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1998
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Abstract :
The gene encoding ADPglucose synthetase (EC fromAgrobacterium tumefacienswas isolated and expressed inEscherichia coli.The recombinant protein was purified to electrophoretic homogeneity in steps including ion-exchange and hydrophobic chromatography. The same purification procedure was utilized to purify ADPglucose synthetase fromA. tumefacienscells. The enzymes from the two sources were purified and characterized and were found to have identical kinetic, regulatory, and structural properties. In polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, only one polypeptide band of 50 kDa was detected. In immunoblotting following electrophoresis, the 50-kDa band reacted with antibodies raised against theEscherichia coliADPglucose synthetase; there was no reaction with antibodies raised against the spinach enzyme. The immunoreactivity of theA. tumefaciensADPglucose synthetase was confirmed in antibody neutralization assays. gel filtration, the native enzyme was shown to be a tetramer. Fructose 6-phosphate and pyruvate were the most effective activators of the enzyme; maximal activation was observed in the ADPglucose synthesis direction, in which the enzyme was activated about ninefold by fructose 6-phosphate and fivefold by pyruvate. Both activators increased the affinity of the enzyme for the substrates ATP and glucose 1-phosphate. Inorganic orthophospate, ADP, AMP, and pyridoxal phosphate behaved as inhibitors of the enzyme. The distinctive regulatory properties of the enzyme fromA. tumefaciensare compared with those of two enterobacterial enzymes and discussed in the context of their deduced amino acid sequences.
Keywords :
Agrobacterium tumefaciens , ADPglucose synthetase , glycogen biosynthesis
Journal title :
Archives of Biochemistry and Biophysics
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