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Title of article :
Enzymatic production of N-acetyl-d-glucosamine from chitin. Degradation study of N-acetylchitooligosaccharide and the effect of mixing of crude enzymes
Author/Authors :
Sashiwa، نويسنده , , Hitoshi and Fujishima، نويسنده , , Shizu and Yamano، نويسنده , , Naoko and Kawasaki، نويسنده , , Norioki and Nakayama، نويسنده , , Atsuyoshi and Muraki، نويسنده , , Einosuke and Sukwattanasinitt، نويسنده , , Mongkol and Pichyangkura، نويسنده , , Rath and Aiba، نويسنده , , Sei-ichi، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
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Abstract :
N-Acetyl-d-glucosamine (GlcNAc) was produced from chitin by use of crude enzyme preparations. The efficient production of GlcNAc by cellulases derived from Trichoderma viride (T) and Acremonium cellulolyticus (A) was observed by HPLC analysis compared to lipase, hemicellulase, and pectinase. β-Chitin showed higher degradability than α-chitin when using cellulase T. The optimum pH of cellulase T was 4.0 on the hydrolysis of β-chitin. The yield of GlcNAc was enhanced by mixing of cellulase T and A.
Keywords :
N-Acetyl-d-glucosamine , Chitin , crude enzyme
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