Title of article :
In vitro Callus Induction and Analysis of Essential Oil Constituents in Leaves and Callus of Pistacia atlantica subsp. kurdica
Aghaei، Peyman نويسنده Department of Agricultural Biotechnology, University of Kurdistan P. O. Box: 416, Sanandaj, Iran , , Bahramnejad، Bahman نويسنده Department of Agricultural Biotechnology, University of Kurdistan P. O. Box: 416, Sanandaj, Iran , , Mozafari، Ali Akbar نويسنده Department of Horticulture, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran ,
Issue Information :
دوفصلنامه با شماره پیاپی 0 سال 2014
In order to optimize callus induction in Pistacia atlantica Desf. subsp. kurdica Rech. f., the effect of different plant growth regulator (PGR) combinations were studied. Inflorescence axis explants were cultured on WPM medium supplemented with 1, 2 and 3 mg/L of TDZ or 1, 2 and 3 mg/L of BA, in combination with 1mg/l different auxins: 2, 4-D, NAA and IBA . Callus initiation was observed in all media evaluated and the highest percentage (100%) was observed in medium containing 1 mg/L BA or TDZ and 1 mg/L NAA. Additionally, anthocyanin, total phenolic compound of callus and immature fruits were measured. Anthocyanin content of callus and immature fruits were 79.42 ± 4.63 and 61.71 ± 3.81 mg cyanidin-3-glucoside/g fresh weights, respectively. Total phenolic content of callus and fruits were 4.91 ± 0.492 and 5.1 ± 0.780 mg gallic acid/g fresh weights. GC-MS analysis of essential oil constituents of P. atlantica subsp. kurdica callus and leaves showed 8 kinds of substances in callus and 13 in leaves. Main composition of callus extract were alpha pinene (89.19%), camphene (1.09%) and Bicyclo[3.1.1] heptane, 6,6-dimethyl-2-methylene (3.40%) and it was alpha Pinene (15.63%), gamma elemene (33.84%) and caryophyllene (9.26%) in leaves.
Journal title :
Journal of Medicinal Plants and By-products